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Antibodies sure to the blocking peptide no more bind to your epitope on the target protein. By evaluating the staining from your blocked antibody compared to the antibody by yourself, you can see which staining is specific.
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This information outlines further more possible optimization of mobile and immunoassay parameters to be certain the best possible results are...
This manual outlines more possible optimization of mobile and immunoassay parameters to be certain the absolute best success are...
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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Blocking peptides are peptides that bind precisely on the focus on antibody and block antibody binding. These peptide commonly has the epitope identified with the antibody.
Goal Details Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions to be a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates for the G1-S phase boundary which is degraded as cells development by way of S stage.
Overexpression of this gene has long been observed in several tumors, which results in chromosome instability, and therefore may lead to tumorigenesis. This protein was located to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial function in endorsing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants more info of this gene, which encode distinctive isoforms, have been explained. Two further splice variants ended up documented but comprehensive nucleotide sequence information is just not still out there.
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